flex polyclonal rabbit anti-human cd3 antibody  (Agilent technologies)

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: Examples of the 500 × 500 pixel CD3 IHC stained images used in the experiments. There were 20 such images. The brown pixels indicate CD3+ T cells while the blue pixels are the CD3− T cells. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques: Staining

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: Flowchart of the CD3+ and CD3− T cells segmentation method.

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques:

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: Scatter plots of two sample CD3+ stained images (a) in (b) RGB and (c) L*a*b* color formats. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques: Staining

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: An example showing (a) a sample image decomposed into (b) CD3+ T cell regions detected by the highlighted in red, (c) CD3− T cell regions highlighted in blue and (d) background regions in white. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques:

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: An evaluation of (a) sensitivity and (b) specificity of CD3+ and CD3− cell segmentation for all four pathologists

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques:

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: Results of computerized CD3+ T cell quantification [based on Eq. (3)] shown against the four pathologists’ quantification estimates. The computerized quantification consistently results within 95% confidence interval of the marked CD3+ T cell population of the four pathologists as shown in (a). It is also observed that the four pathologists exhibit a consistent and predictable pattern in marking CD3+ and CD3− T cells. A different pattern is seen in (b) which shows results of CD3+ T cell population estimation through “eyeballing.” For visual clarity, both data in (a) and (b) are sorted in ascending order to the mean percentage of CD3+ T cell population in (a). [Color figure can be viewed at wileyonlinelibrary.com.]

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques:

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: Performance of pathologists and the computer in determining CD3+ T cell coverage

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques:

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Article Title: Computer-assisted Quantification of CD3+ T Cells in Follicular Lymphoma

doi: 10.1002/cyto.a.23049

Figure Lengend Snippet: The Bland-Altman plots illustrating the relationships between the “eyeballing” CD3+ T cells estimates and the average cell marking measurements for all four pathologists shown in (a) through (d). The computerized quantification is compared to the average cell marking measurement and shown in (e). The plots represent bias versus (pA +pn)/2. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Polyclonal rabbit anti-human CD3 antibody was used from Dako A0452 at 1:400 dilution.

Techniques: